Thermo Fisher Scientific MLH1 Monoclonal Antibody (164C819)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-3 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

164C819

Immunogen

A synthetic peptide corresponding to amino acids 387-403 DSREQKLDAFLQPLSKP of human MLH1 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:MLH1,uniProtId:P40692-1,ncbiNodeId:9606,antigenRange:387-403,antigenLength:756,antigenImageFileName:MA1-41246_MLH1_P40692-1_House_mouse.svg,antigenImageFileNamePDP:MA1-41246_MLH1_P40692-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1076868

Product Specific Information

This peptide sequence is 93% homologous to mouse and dog MLH1.

Suggested positive control: Daudi cell lysate.

Target Information

MLH1 is a DNA mismatch repair protein. The repair of mismatch DNA is essential to maintaining the integrity of genetic information over time. An alteration of microsatellite repeats is the result of slippage owing to strand misalignment during DNA replication and is referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been related to human carcinogenesis. The importance of mismatch repair genes became apparent with the identification of the genetic basis for hereditary nonpolyposis colon cancer (HNPC). MSHS2 is involved in the initial cognition of mismatch nucleotides during the replication mismatch repair process. It is thought that after MSH2 binds to a mismatched DNA duplex it is joined by a heterodimer of MLH1 and PMSH, which together help facilitate the later steps in mismatch repair.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.