Thermo Fisher Scientific APIP Monoclonal Antibody (19F461)
APIP 단백질을 인식하는 마우스 단클론 항체로, Western blot과 IHC(P) 분석에 적합합니다. 인간 및 마우스 시료에 반응하며, 단백질 G로 정제된 액상 형태입니다. 단기 4°C, 장기 -20°C 보관 권장. 연구용으로만 사용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 19F461 |
| Immunogen | Recombinant APIP protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein G |
| Storage buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1070436 |
Product Specific Information
- Suggested positive control: HeLa or 293 cells, or antigen standard for APIP (transient overexpression lysate).
Target Information
The mammalian homologues of the key cell death gene CED-4 in C. elegans have been identified from human and mouse and designated Apaf 1 (apoptosis protease-activating factor 1).
Apaf 1 binds to cytochrome c (Apaf 2) and Caspase-9 (Apaf 3), leading to Caspase-9 activation. Activated Caspase-9 subsequently cleaves and activates Caspase-3, a key protease responsible for proteolytic cleavage of many proteins during apoptosis.
Cho et al. identified a new Apaf 1 Interacting Protein (APIP, also known as CG129 and MMRP19) as a negative regulator of ischemic injury. APIP competes with Caspase-9 for binding to Apaf 1.
APIP encodes a 204 amino acid protein, while its isoform APIP2 encodes a 242 amino acid protein differing in its N-terminus due to alternative splicing.
APIP transcript is ubiquitously expressed in most adult tissues, with high expression in skeletal muscle, heart, and kidney.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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