Thermo Fisher Scientific APIP Monoclonal Antibody (19F461)

Applications

Product Specifications

Product Specific Information

• Suggested positive control: HeLa or 293 cells, or antigen standard for APIP (transient overexpression lysate).

Target Information

The mammalian homologues of the key cell death gene CED-4 in C. elegans have been identified from human and mouse and designated Apaf 1 (apoptosis protease-activating factor 1).
Apaf 1 binds to cytochrome c (Apaf 2) and Caspase-9 (Apaf 3), leading to Caspase-9 activation. Activated Caspase-9 subsequently cleaves and activates Caspase-3, a key protease responsible for proteolytic cleavage of many proteins during apoptosis.

Cho et al. identified a new Apaf 1 Interacting Protein (APIP, also known as CG129 and MMRP19) as a negative regulator of ischemic injury. APIP competes with Caspase-9 for binding to Apaf 1.
APIP encodes a 204 amino acid protein, while its isoform APIP2 encodes a 242 amino acid protein differing in its N-terminus due to alternative splicing.
APIP transcript is ubiquitously expressed in most adult tissues, with high expression in skeletal muscle, heart, and kidney.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.