Thermo Fisher Scientific GFP Monoclonal Antibody (9F9.F9), AP
GFP 단백질을 인식하는 Thermo Fisher Scientific의 Mouse Monoclonal 항체(AP 결합형). Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. 높은 특이성과 안정성으로 연구용으로 최적화. 4°C 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (IHC) | 1:200–1:1,000 |
| ELISA | 1:2,000–1:12,000 |
| Immunoprecipitation (IP) | 1:500–1:2,500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9F9.F9 |
| Immunogen | Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP-containing fusion protein corresponding to the full-length amino acid sequence (246aa) derived from Aequorea victoria. |
| Conjugate | Alkaline Phosphatase (AP) |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.05M Tris HCl (pH 8) with 50% glycerol, 10 mg/mL BSA, 0.15M NaCl, 0.0001M ZnCl₂, 0.001M MgCl₂ |
| Contains | 0.01% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Available Formats
- Unconjugated
- Biotin
- DyLight 405, 488, 549, 649, 680, 800
- FITC
- HRP
- Rhodamine
- Custom conjugation available upon request
Product Specific Information
- Store vial at 4°C before opening. Do not freeze.
- Stable at 4°C as an undiluted liquid.
- Dilute only prior to immediate use.
- Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
- Immunoelectrophoresis shows a single precipitin arc against anti-Mouse Serum, anti-Alkaline Phosphatase, purified and partially purified GFP (Aequorea victoria).
- Reactivity observed against recombinant GFP by Western blot and ELISA; no cross-reactivity with RFP.
Target Information
Green Fluorescent Protein (GFP) from Aequorea victoria emits green light during bioluminescence. GFP is widely used as a reporter protein for gene expression and as a fusion tag in molecular and cellular biology.
It enables visualization of protein localization, movement, and subcellular dynamics in living cells.
GFP is a 27 kDa monomeric protein that forms a fluorescent pigment autonomously. It absorbs blue light (395 nm) and emits green light (508 nm) without the need for additional cofactors.
GFP fluorescence is stable and species-independent, suitable for various applications including:
- Gene expression and protein localization studies
- Protein-protein interaction assays (yeast two-hybrid system)
- Fluorescence resonance energy transfer (FRET) experiments
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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