Thermo Fisher Scientific Granzyme H Polyclonal Antibody
Human Granzyme H를 인식하는 Goat Polyclonal Antibody로, WB, ICC/IF, IP에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, Lyophilized 형태로 제공. Pro 및 active form 모두 검출 가능하며, 낮은 endotoxin 수준 유지.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–15 µg/mL |
| Immunoprecipitation (IP) | 25 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant human Granzyme H (Glu19–Leu246) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609230 |
Product Specific Information
This antibody detects both the pro and active forms of human Granzyme H in direct ELISAs and Western blots.
In direct ELISAs, approximately 25% cross-reactivity with recombinant human Granzyme B is observed and approximately 5% cross-reactivity with recombinant mouse Granzyme C, D, and G is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endotoxin level is <0.01 EU per 1 µg of the antibody by the LAL method.
Target Information
This gene encodes a member of the peptidase S1 family of serine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate a chymotrypsin-like protease.
This protein is reported to be constitutively expressed in the NK (natural killer) cells of the immune system and may play a role in the cytotoxic arm of the innate immune response by inducing target cell death and by directly cleaving substrates in pathogen-infected cells.
This gene is present in a gene cluster with another member of the granzyme subfamily on chromosome 14.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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