Thermo Fisher Scientific PICK1 Polyclonal Antibody
PICK1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC, Flow Cytometry에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 Affinity chromatography 정제, 액상 형태로 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within rat PICK1 aa 280–330 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | TBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2913264 |
Product Specific Information
- Positive Control: SH-SY5Y cell lysate, 293 cell lysate, F9, MCF-7, rat brain tissue, human colon tissue, mouse brain tissue
- Subcellular Location: Cytoskeleton, perinuclear region, membrane, postsynaptic density, synaptosome
Target Information
PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK1 functions as a targeting and transport protein and interacts with protein kinase C alpha (PKCα), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain.
The interaction of PICK1 with PKCα is highly dependent on kinase activation. PICK1 directs the activated form of PKCα to membrane-anchored GluR2. Once phosphorylated by PKCα, GluR2 is released from synaptic anchor proteins and transported from the synaptic membrane in a PICK1-dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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