Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Violet 421, eBioscience

Thermo Fisher Scientific CD45 Monoclonal Antibody (30-F11), Brilliant Violet 421, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 532
• Alexa Fluor 561
• Alexa Fluor 660
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV Series (395–805)
• Brilliant Violet Series (480–786)
• eFluor Series (450, 506)
• FITC
• Functional Grade
• NovaFluor Series (Blue, Red, Violet, Yellow)
• PE and derivatives (PE-Cyanine5, PE-Cyanine7, etc.)
• PerCP-Cyanine5.5
• Super Bright Series (436–780)

Product Specific Information

The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45 (Leukocyte Common Antigen, LCA). CD45 is expressed on all hematopoietic cells except mature erythrocytes and platelets. It possesses tyrosine phosphatase activity and plays a key role in lymphocyte activation.

Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse bone marrow cells. Use ≤ 0.25 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best performance.

Fluorochrome Information:
Brilliant Violet™ 421 emits at 423 nm and is suitable for cytometers equipped with a 405 nm violet laser.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed on all nucleated hematopoietic cells, comprising ~10% of lymphocyte surface proteins. It regulates Src-family kinases and is essential for T- and B-cell receptor signaling. CD45 isoforms vary by alternative splicing and cell type, influencing membrane localization and signaling activity. It also modulates integrin-mediated pathways and cytokine receptor signaling, contributing to immune regulation and cell survival.

For Research Use Only. Not for use in diagnostic procedures.

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