Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Brilliant Violet 421, eBioscience
마우스 CD31(PECAM-1)을 인식하는 단클론 항체로, Brilliant Violet 421 형광체가 결합되어 있습니다. 유세포분석(Flow Cytometry)에 적합하며, 0.06 µg/test 농도로 권장됩니다. 4°C 암소 보관, 동결 금지. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Flow Cytometry (Flow) | 0.06 µg/test |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Violet™ 421, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 390 |
| Conjugate | Brilliant Violet™ 421 |
| Excitation / Emission Max | 406 / 423 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2929069 |
Available Formats
- Unconjugated
- APC
- Biotin
- Brilliant UV 661
- Brilliant UV 737
- Brilliant Violet 711
- eFluor 450
- FITC
- Functional Grade
- NovaFluor Blue 660-120S
- NovaFluor Red 685
- NovaFluor Red 710
- NovaFluor Red 725
- NovaFluor Red 755
- NovaFluor Yellow 610
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Request custom conjugation
Product Specific Information
Description:
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on leukocytes and platelets. CD31 plays roles in adhesion, cell-cell and cell-matrix interactions, and signal transduction.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.06 µg/test (100 µL final volume). Optimal dilution should be determined empirically.
Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is suitable for cytometers with a 405 nm violet laser.
Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.
Excitation / Emission / Laser:
407 nm / 423 nm / Violet Laser
Trademark Notice:
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.
Target Information
CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs that recruit protein-tyrosine phosphatases upon phosphorylation. It is expressed throughout the vascular system, mainly at intercellular junctions. CD31 mediates homophilic binding and is involved in cell adhesion, migration, angiogenesis, apoptosis, and immunoregulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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