Thermo Fisher Scientific CD31 (PECAM-1) Monoclonal Antibody (390), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 711
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

Description:
The 390 monoclonal antibody reacts with mouse CD31 (PECAM-1, gpIIa), a 130–140 kDa surface protein expressed by endothelial cells and at low levels on leukocytes and platelets. CD31 plays roles in adhesion, cell-cell and cell-matrix interactions, and signal transduction.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse bone marrow cells. Recommended ≤0.06 µg/test (100 µL final volume). Optimal dilution should be determined empirically.

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is suitable for cytometers with a 405 nm violet laser.

Staining Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific interactions.

Excitation / Emission / Laser:
407 nm / 423 nm / Violet Laser

Trademark Notice:
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company. Powered by Sirigen™.

Target Information

CD31 (PECAM-1) is an inhibitory coreceptor regulating T and B cell signaling via ITIM motifs that recruit protein-tyrosine phosphatases upon phosphorylation. It is expressed throughout the vascular system, mainly at intercellular junctions. CD31 mediates homophilic binding and is involved in cell adhesion, migration, angiogenesis, apoptosis, and immunoregulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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