Thermo Fisher Scientific CD45.2 Monoclonal Antibody (104), Brilliant Violet 421, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 532
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395
• Brilliant UV 496
• Brilliant UV 563
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 605
• Brilliant Violet 650
• Brilliant Violet 711
• Brilliant Violet 786
• eFluor 450
• eFluor 506
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 555
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• PE
• PE-Cyanine7
• PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 780
• Request custom conjugation

Product Specific Information

The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. Strains expressing CD45.2 include C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A, and AKR. CD45.2 is expressed by all leukocytes in these strains.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse splenocytes. Recommended ≤1.0 µg/test in 100 µL final volume. Optimize titration per assay.

Fluorochrome Information:
Brilliant Violet™ 421 (BV421) emits at 423 nm and is suitable for cytometers equipped with a violet (405 nm) laser. Ensure compatibility with your instrument.

When using multiple polymer dye-conjugated antibodies (e.g., Brilliant Violet™, Super Bright™), it is recommended to use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42) or
• Brilliant Stain Buffer™ (Product # 00-4409-75)

Excitation: 407 nm
Emission: 423 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company and used under license. Powered by Sirigen™.

Target Information

CD45.2 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase expressed in all nucleated hematopoietic cells, comprising about 10% of surface proteins in lymphocytes. It regulates Src-family kinases, influences lymphocyte development and antigen signaling, and exists in multiple isoforms due to alternative splicing. CD45.2 also modulates integrin-mediated signaling and contributes to apoptosis regulation. CD45RA is one isoform with distinct expression among lymphoid subtypes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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