Thermo Fisher Scientific GNE Polyclonal Antibody
Human GNE 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human GNE. Recombinant protein control fragment (Product # RP-89765) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2642085 |
Product Specific Information
Immunogen sequence:
VPFDQFIQLV AHAGCMIGNS SCGVREVGAF GTPVINLGTR QIGRETGENV LHVRDADTQD KILQALHLQF GKQYPCSKIY GDGNAVPRIL KFLKSIDLQE PLQKKFCFPP VKENISQDID HILETLSALA VDLGGTNLRV A
Highest antigen sequence identity to the following orthologs:
- Mouse: 100%
- Rat: 100%
Target Information
GNE is a bifunctional enzyme that initiates and regulates the biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. It acts as a rate-limiting enzyme in the sialic acid biosynthetic pathway. Sialic acid modification of cell surface molecules is crucial for their function in processes such as cell adhesion and signal transduction. Differential sialylation of cell surface molecules is also implicated in tumorigenicity and metastatic behavior of malignant cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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