Thermo Fisher Scientific TIMD4 Recombinant Rat Monoclonal Antibody (5G3)

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

Assay-dependent

-

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG1, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

5G3

Immunogen

This antibody was raised by immunising female Lewis rats (Harlan Sprague-Dawley) with Tim-4:Ig fusion protein in complete Freund`s adjuvant.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074601

Product Specific Information

Specificity: This antibody is specific for murine TIM-4, which unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen presenting cells such as lymphoid and myeloid DC`s and macrophages.

Target Information

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. TIM-4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM-1, and this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM-4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain. The TIM-4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM-1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.