
Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
염소 유래 Rabbit IgG(H+L) 교차흡착 2차 항체로 DyLight 550 형광 표지. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. 최소한의 비특이 반응으로 높은 신뢰성 제공. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 550
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rabbit IgG-heavy and light chain |
| Conjugate | DyLight™ 550 |
| Excitation / Emission Max | 556 / 570 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556613 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, horse, human, mouse, pig and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimum reactivity to bovine, chicken, horse, human, mouse, pig and rat.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit targets.
Secondary antibodies enhance versatility and sensitivity by enabling multiple detection systems (e.g., HRP, AP, fluorescence) and signal amplification through multiple binding events to primary antibodies.
Typically, these antibodies are generated by immunizing the host with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
DyLight™, Alexa Fluor™, FITC, HRP, Biotin, APC, PE, TRITC, Rhodamine, Texas Red 등 다양한 형광 및 효소 표지 옵션 제공.
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