Thermo Fisher Scientific Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 755
DyLight 755 형광 표지된 염소 유래 항-토끼 IgG (H+L) 2차 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. 교차 흡착 처리로 비특이적 반응 최소화. 760/789 nm 파장에서 최적 형광. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rabbit IgG-heavy and light chain |
| Conjugate | DyLight™ 755 |
| Excitation / Emission Max | 760 / 789 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556615 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, horse, human, mouse, pig and rat |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, horse, human, mouse, pig, and rat proteins, ensuring high specificity in immunoassays.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins. They are widely used for detection, sorting, or purification of rabbit antibodies.
Secondary antibodies provide versatility for multiple detection systems (e.g., HRP, AP, fluorescence) and greater sensitivity through signal amplification, as multiple secondary antibodies can bind to one primary antibody.
These antibodies are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, or label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Available Conjugates
This antibody is also available conjugated with various fluorophores and labels, including Alexa Fluor™, DyLight™, FITC, HRP, PE, APC, and others. Custom conjugation is available upon request.
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