Thermo Fisher Scientific APIP Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:1,000

Product Specifications

Product Specific Information

• Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji
• Predicted reactivity: Mouse (94%), Zebrafish (82%), Xenopus laevis (82%), Chicken (87%), Bovine (91%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The mammalian homologues of the key cell death gene CED-4 in C. elegans have been identified from human and mouse and designated Apaf 1 (apoptosis protease-activating factor 1).
Apaf 1 binds to cytochrome c (Apaf 2) and Caspase-9 (Apaf 3), leading to Caspase-9 activation. Activated Caspase-9 in turn activates Caspase-3, a key protease responsible for the proteolytic cleavage of many proteins in apoptosis.

Recently, Cho et al. identified a new Apaf 1 interacting protein (APIP, also known as CG129 and MMRP19), which acts as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf 1.
APIP encodes a 204 amino acid protein, while its isoform APIP2 encodes a 242 amino acid protein, differing in the N-terminus due to alternative splicing.
APIP transcript is ubiquitously expressed in most adult tissues, with high levels in skeletal muscle, heart, and kidney.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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