Thermo Fisher Scientific ErbB4 Polyclonal Antibody
ErbB4 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, IP 등에 사용 가능. Human, Mouse, Rat에 반응하며, 항원 친화 크로마토그래피로 정제됨. PBS buffer에 20% glycerol 포함, 4°C 또는 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| Immunoprecipitation (IP) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 850–1101 of Human ERBB4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.97 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2546748 |
Product Specific Information
- Recommended positive controls: A431, mouse brain, rat brain
- Predicted reactivity: Mouse (89%), Rat (97%), Xenopus laevis (86%), Chicken (88%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Oncogene ERBB4 (HER4) is a member of the type I receptor protein tyrosine kinase subfamily that includes EGFR, ERBB2, and ERBB3. ERBB4 binds and is activated by neuregulins and other factors, inducing various cellular responses such as mitogenesis and differentiation.
ErbB4 is a receptor for heregulin and mediates HGL-stimulated tyrosine phosphorylation of itself. It plays a key role in signal transduction regulating metabolism, transcription, cell cycle progression, cytoskeletal rearrangement, apoptosis, and differentiation.
ErbB4 is predominantly expressed in several breast carcinoma cell lines and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Expression levels vary among different breast tumor cell lines, suggesting its potential role in certain malignancies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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