Thermo Fisher Scientific Vimentin Monoclonal Antibody (GT7812)
Vimentin 단백질을 표적하는 Mouse IgG2a 단클론 항체로, Human, Mouse, Rat에 반응합니다. WB, IHC, ICC, ELISA 등 다양한 응용에 적합하며, 고순도 Protein G 정제 제품입니다. 세포 구조 및 발달 연구용으로 권장됩니다.
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Thermo Fisher Scientific Vimentin Monoclonal Antibody (GT7812)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
| ELISA | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GT7812 |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Vimentin |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.64 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787117 |
Product Specific Information
- Keep as concentrated solution.
- Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (85%), Dog (98%), Chicken (92%), Chimpanzee (99%), Bovine (98%), Guinea pig (97%).
- Positive Control: 293T, HeLa, C8D30, NIH-3T3, C2C12, U87-MG, SK-N-SH, IMR32, SK-N-AS, NT2D1, rat brain, HEB, A549, H358, MCF10A.
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal changes facilitating cholesterol transport to mitochondria, enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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