Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), PE, eBioscience
인간 CD19 항원에 특이적인 HIB19 단클론 항체로, PE 형광체가 결합된 형태입니다. Flow Cytometry 분석용으로 최적화되어 있으며, 5 µL/test로 사용 가능합니다. B세포 발달 단계 연구 및 면역표현형 분석에 적합합니다. 4°C 암실 보관, 동결 금지.
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Thermo Fisher Scientific CD19 Monoclonal Antibody (HIB19), PE, eBioscience™
Applications
- Flow Cytometry (Flow)
Tested Dilution: 5 µL (0.25 µg)/test
Publications: View 47 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HIB19 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS (pH 7.2) with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1834377 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV (395, 563, 615, 661, 737)
- Brilliant Violet (480, 605, 650, 711)
- eFluor 450, 506
- FITC
- Functional Grade
- NovaFluor (Blue, Red, UV, Violet, Yellow series)
- PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright (436, 600, 645, 702, 780)
- Request custom conjugation available
Product Specific Information
The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein expressed by B cells during all developmental stages except terminally differentiated plasma cells. Follicular dendritic cells also express CD19. CD19 forms a multimolecular complex with CD21, CD81, Leu13, and MHC class II, associating with BCR to induce tyrosine phosphorylation, calcium flux, and B cell proliferation. The SJ25C1 and HIB19 antibodies recognize overlapping epitopes.
Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells.
Recommended Use: 5 µL (0.25 µg) per test in a final volume of 100 µL. Cell number may range from 10⁵–10⁸ cells/test.
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD19 is a member of the immunoglobulin superfamily with two Ig-like domains. It is expressed on 100% of peripheral B cells and some myeloid leukemia cells. CD19 acts as a signal-amplifying coreceptor for BCR, regulating normal and malignant B cell proliferation. Mutations in the CD19 gene can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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