Thermo Fisher Scientific CD305 (LAIR1) Monoclonal Antibody (NKTA255), NovaFluor Yellow 700, eBioscience

Thermo Fisher Scientific CD305 (LAIR1) Monoclonal Antibody (NKTA255), NovaFluor Yellow 700

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.6 µg)/test
Publications: -

Product Specifications

Additional Formats

• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 660
• PE
• Request custom conjugation

Product Specific Information

This NKTA255 monoclonal antibody reacts with human CD305 (LAIR1), a type I transmembrane glycoprotein possessing an extracellular Ig-like domain and two intracellular ITIMs. CD305 is expressed on most immune cells (T, NK, B cells, monocytes, dendritic cells, eosinophils, basophils, mast cells) and CD34+ progenitor cells.
It functions as an inhibitory receptor, blocking cytokine-mediated signaling, proliferation, and apoptosis. Collagens act as ligands for CD305.

The NKTA255 antibody has been reported to inhibit NK cytolytic activity.
Applications Reported: Flow cytometric analysis of human peripheral blood cells.
Applications Tested: Use at 5 µL (0.6 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test).

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability testing.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails/master mixes before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures engineered for optimized fluorescent signatures minimizing spillover and spread.

Excitation: 552 nm
Emission: 700 nm
Laser: 561 nm (Yellow Laser)

Target Information

LAIR1 (CD305) functions as an inhibitory receptor regulating cytolytic activity of NK, B, and T cells.
Activation via Tyr phosphorylation recruits phosphatases PTPN6 and PTPN11, reducing intracellular calcium increase upon B-cell receptor ligation. It modulates cytokine production, down-regulating IL2 and IFNG, while inducing TGF-β secretion.
In B-cells, it suppresses IgG/IgE production and inhibits IL8, IL10, and TNF secretion.
LAIR1 inhibits proliferation and induces apoptosis in myeloid leukemia cell lines and prevents NF-κB p65 nuclear translocation and I-κBα phosphorylation.
It also inhibits differentiation of peripheral blood precursors toward dendritic cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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