Thermo Fisher Scientific CYP2A6 Monoclonal Antibody (OTI2A8), TrueMAB
인간 CYP2A6 단백질을 인식하는 마우스 단클론 항체로, WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. 무보존제, 동결건조 형태로 제공되며, 재구성 후 1 mg/mL 농도로 사용 가능. 약물 대사 관련 연구에 활용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2A8 |
| Immunogen | Full length human recombinant protein of human CYP2A6 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
CYP2A6 encodes a member of the cytochrome P450 superfamily of enzymes. These monooxygenases catalyze reactions involved in drug metabolism and synthesis of cholesterol, steroids, and other lipids.
This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. It hydroxylates coumarin and metabolizes nicotine, aflatoxin B1, nitrosamines, and certain pharmaceuticals.
Individuals with specific allelic variants may exhibit poor metabolizer phenotypes. CYP2A6 is part of a large cluster of cytochrome P450 genes (CYP2A, CYP2B, CYP2F) on chromosome 19q. Formerly referred to as CYP2A3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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