Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific IL-17A Monoclonal Antibody (eBio17B7), Brilliant Ultra Violet 805, eBioscience™

Applications

• Flow Cytometry (Intracellular staining)

Tested Dilution: 0.5 µg/test

Product Specifications

Additional Available Formats

• Alexa Fluor 488
• APC
• Biotin
• Brilliant UV 395, 615, 661, 737
• Brilliant Violet 421, 650, 711, 786
• eFluor 450, 506, 660
• FITC
• PE, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5
• Request custom conjugation

Product Specific Information

The eBio17B7 antibody reacts with mouse and rat IL-17A with no recognition of IL-17F.
IL-17A is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection.
IL-23-dependent, IL-17A-producing CD4+ T cells (Th17 cells) represent a unique subset distinct from Th1 and Th2 cells.

Applications Reported:
Used for intracellular staining followed by flow cytometric analysis.

Applications Tested:
Validated using stimulated mouse splenocytes with Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤ 0.5 µg/test.

Brilliant Ultra Violet™ 805 (BUV805):

• Tandem dye emitting at 797 nm, excited by 355 nm UV laser
• Compatible with cytometers equipped with ultraviolet laser
• Sensitive to photo-oxidation; protect from light
• Compatible with short-term methanol fixation (≤1 hour)
• Excitation: 355 nm; Emission: 797 nm

For multi-color panels, use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize non-specific polymer interactions.

Fixation:
Samples can be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses and is implicated in autoimmune diseases.
IL-17A is a 32 kDa disulfide-linked homodimer of 136 amino acids, signaling through IL-17R/CDw217.
It enhances ICAM-1 expression and stimulates secretion of IL-6, IL-8, G-CSF, and PGE2 from epithelial and fibroblastic cells.
IL-17A regulates NF-κB and MAPK pathways and promotes nitric oxide production.

For Research Use Only. Not for use in diagnostic procedures.

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