Thermo Fisher Scientific Phospho-Btk (Tyr223, Tyr225) Polyclonal Antibody
BTK 단백질의 Tyr223/225 인산화 상태를 특이적으로 인식하는 rabbit polyclonal antibody. Western blot 및 IHC(P)에서 사용 가능하며, 인간, 생쥐, 랫드 반응성. 1 mg/mL 농도의 액상 형태로 제공되며, PBS/glycerol buffer에 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human BTK (Accession Q06187), corresponding to amino acid residues around phosphorylated Tyr223. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817047 |
Product Specific Information
This antibody detects endogenous levels of BTK only when phosphorylated at Tyr223/225.
Target Information
Bruton tyrosine kinase (BTK) is a cytoplasmic tyrosine kinase belonging to the SRC-related TEC subfamily of tyrosine kinases.
Mutations in the BTK gene are associated with severe developmental blocks in human B-cell ontogeny and immunodeficiency disorders.
BTK interacts with members of the toll-like receptor (TLR) family, including TLR4, TLR6, TLR8, and TLR9, which are critical in innate and adaptive immunity.
It also interacts with key proteins in TLR4 signal transduction such as MyD88, TIRAP, and IRAK, but not TRAF-6, indicating its role in lipopolysaccharide signal transduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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