Thermo Fisher Scientific RTN1 Monoclonal Antibody (MON161)
RTN1 단백질을 인식하는 Thermo Fisher Scientific의 MON161 단일클론 항체로, 인간·생쥐·햄스터·랫드 시료에 반응합니다. Western blot, IHC, ICC/IF, Flow Cytometry에 사용 가능하며, 135 kDa reticulon-1A 검출에 적합합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:50–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:100 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Hamster, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MON161 |
| Immunogen | Partially purified bacterially expressed reticulon-1A (NSP-A) hybrid protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2183567 |
Product Specific Information
MA1-06811 detects reticulon-1A (NSP-A) in human, mouse, hamster, and rat samples.
It has been successfully used in Western blotting, FACS, immunocytochemistry, and immunohistochemistry procedures.
By Western blot, it detects a ~135 kDa band representing reticulon-1A.
The immunogen is a partially purified bacterially expressed reticulon-1A (NSP-A) hybrid protein.
Target Information
Reticulon-1 (formerly NSP, Neuroendocrine-Specific-Protein) is specifically expressed in neural and neuroendocrine tissues.
The NSP gene is located on human chromosome 14q21–q22 and encodes three overlapping proteins: reticulon-1A (NSP-A), reticulon-1B (NSP-B), and reticulon-1C (NSP-C).
These proteins are anchored to endoplasmic reticulum membranes through their common C-terminal regions.
- Reticulon-1A: ~135 kDa protein, occurs in various isoforms depending on serine phosphorylation; a reliable marker for neuroendocrine differentiation in lung cancer diagnosis.
- Reticulon-1B: 45 kDa phosphoprotein, found only in lung cancer cell line NCI-H82.
- Reticulon-1C: 23 kDa non-phosphorylated protein, co-expressed with Reticulon-1A in small cell lung carcinoma (SCLC) cell lines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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