Thermo Fisher Scientific IkB alpha Polyclonal Antibody
Rabbit polyclonal antibody recognizing human IkB alpha, an NF-κB inhibitor protein. Validated for WB, IHC(F), ELISA, and IP. Suitable for detecting NF-κB signaling pathway components. For research use only.
- 카탈로그번호
- PA185928
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific IkB alpha Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:1,000–1:5,000 |
| ELISA | 1:20,000–1:100,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NFkBIa peptide corresponding to a region near the C-terminus of the human protein, conjugated with KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage conditions | 4°C or -20°C if preferred |
| Shipping conditions | Wet ice |
| RRID | AB_2235944 |
Product Specific Information
Rabbit anti-human NFκB inhibitor alpha antibody recognizes NF-kappa-B inhibitor alpha (IkB alpha), one of seven members of the IkB class of inhibitor proteins identified to date.
Target Information
IkB-alpha is a 40 kDa protein that inhibits NF-kappaB activity by binding NF-kappaB dimers in the cytosol, masking their nuclear localization sequences, and preventing nuclear translocation. Upon stimulation by cytokines (TNF-α, IL-1β), chemokines, PMA, growth factors, LPS, UV irradiation, viral infection, or stress, IkB-alpha is degraded, liberating NF-kappaB for gene activation.
The human gene is located on chromosome 14q. Activation of NFkB requires phosphorylation of IkB on serine residues 32 and 36 by IkB kinase alpha (IKKα, also known as CHUK). IKKα interacts with IkB-alpha and is essential for NFkB activation in response to proinflammatory cytokines. The IKK complex, composed of IKKα, IKKβ, and IKKγ, is regulated by NFkB-inducing kinase (NIK), a key mediator of NFkB pathway activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.