Thermo Fisher Scientific TOP3A Polyclonal Antibody

Thermo Fisher Scientific TOP3A Polyclonal Antibody

Applications

• Western Blot (WB)

Tested Dilution: 1:500–1:1,000
Publications: [References available upon request]

Product Specifications

Product Specific Information

The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.

Target Information

DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA by transiently breaking and rejoining DNA strands.

• Topoisomerase I (Topo I) and Topoisomerase II (Topo II) relax both positive and negative supercoils.
• Topoisomerase III (Topo III) relaxes only negative supercoils.
• Topo IIIα exists as long and short isoforms produced by alternative splicing. It localizes to the nucleolus and remains highly expressed throughout the cell cycle in HL-60 cells.
• Topo IIIβ exists as three isoforms (β-1, β-2, β-3), also produced by alternative splicing.
  • Topo IIIβ-1: expressed in testis, heart, and skeletal muscle
  • Topo IIIβ-2: expressed in thymus, kidney, and pancreas

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.