Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Blue 530, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 4 µL (0.6 µg)/test

Product Specifications

Additional Available Formats

• APC-eFluor 780
• Functional Grade
• NovaFluor Blue 510 / 585 / 610-70S / 660-120S / 660-40S / 760
• NovaFluor Red 685 / 700 / 725 / 755
• NovaFluor Yellow 590 / 610 / 660 / 690 / 700 / 730 / 755
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation

Product Specific Information

This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (Fc gamma RIII), which exists as two isoforms: FcγRIIIA and FcγRIIIB.

• FcγRIIIA: Expressed on monocytes, macrophages, and NK cells
• FcγRIIIB: Expressed on neutrophils

Binding of IgG to CD16 induces signal transduction pathways leading to ADCC, phagocytosis, cytokine release, and antigen presentation.

• Clone 3G8 recognizes the same epitope as CB16 but a distinct one from B73.1.
• Each product contains one vial of NovaFluor conjugate and one vial of CellBlox Plus Blocking Buffer.
• Reported application: Flow cytometric analysis.
• Tested application: 4 µL (0.6 µg) per 100 µL test volume (10⁵–10⁸ cells/test).

Important Notes

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• Use CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls, use 5 µL per 100 µL cell sample.

NovaFluor conjugates utilize Phiton™ technology with engineered fluorescent signatures optimized for reduced spillover and spread.
Excitation: 509 nm; Emission: 530 nm; Laser: 488 nm (Blue).

Target Information

CD16 (FCGR3A) encodes a receptor for the Fc portion of IgG, mediating immune complex clearance, ADCC, and modulation of immune responses.

• Expressed on NK cells (membrane-anchored form) and neutrophils (PI-anchored form via FCGR3B).
• Mutations are associated with Immunodeficiency 20, recurrent viral infections, systemic lupus erythematosus, and neonatal neutropenia.
• Multiple alternatively spliced isoforms exist.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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