Thermo Fisher Scientific CD42b Monoclonal Antibody (HIP1), PE-Cyanine7, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

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Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

HIP1

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

View additional formats

• Unconjugated
• APC
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 610
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Request custom conjugation

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2762447

Product Specific Information

Description: The HIP1 monoclonal antibody reacts with the human CD42b molecule, a 135 kDa transmembrane protein also known as platelet gpIb.

Applications Reported: This HIP1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HIP1 antibody has been pre-diluted and tested by flow cytometric analysis of human platelets. This may be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

CD42b is a platelet activation marker involved in the process of coagulation as an aggregating factor. It interacts with extracellular matrix as well as with adhesion molecules. CD42b expression and activation seems to be regulated by VEGF and PDGF. CD42b composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis. Further, CD42b antigen is expressed on platelets and on megakaryocytes in bone marrow. Several polymorphisms and mutations have been described in the CD42b, some of which are the cause of Bernard-Soulier syndromes and platelet-type von Willebrand disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.