Thermo Fisher Scientific PIP5K2B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (IHC)

1:100-1:500

-

ELISA (ELISA)

1:20,000-1:60,000

-

Immunoprecipitation (IP)

0.2 µg/mL

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PIP5K2B affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of human PIP5K2B. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PI4K2B,uniProtId:Q8TCG2-1,ncbiNodeId:9606,antigenRange:481,antigenLength:481,antigenImageFileName:600-401-J28_PI4K2B_Q8TCG2-1_Rabbit.svg,antigenImageFileNamePDP:600-401-J28_PI4K2B_Q8TCG2-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.65 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 50% glycerol, 0.15M NaCl

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-PIP5K2B specific towards PIP5K2B. A BLAST analysis was used to suggest cross-reactivity with Human, Mouse, Rat, Chicken, and Primate based on 100% sequence homology. Cross-reactivity with PIP5K2B from other sources has not been determined.

Target Information

PI4K2B lipid kinase is a member of the phosphatidylinositol 4-kinase family (PI4K) that phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate which are immediate precursor of several important signaling and scaffolding molecules. PI4K2B is primarily a cytosolic PI4K that is recruited to membranes where it stimulates phosphatidylinositol 4,5-bisphosphate syntheses. PI4K2B uses phosphatidylinositol as its primary substrate and has no activity on phosphatidylinositol monophosphates. PI4K2B is a restricted minor histocompatibility antigen in patients that had been successfully treated with donor lymphocyte infusions for relapsed chronic myeloid leukemia after allogeneic stem cell transplantation. Phosphatidylinositol 4-kinases (PI4Ks) phosphorylate phosphatidylinositol to generate phosphatidylinositol 4-phosphate (PIP), an immediate precursor of several important signaling and scaffolding molecules. PIP itself may also have direct functional and structural roles. PI4K2B is a primarily cytosolic PI4K that is recruited to membranes, where it stimulates phosphatidylinositol 4, 5-bisphosphate synthesis (Wei et al., 2002 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.