Thermo Fisher Scientific POLE3 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:3,000

Product Specifications

Product Specific Information

• Positive Control: 293T, A431, HeLa, HepG2
• Predicted Reactivity: Mouse (98%), Rat (100%), Zebrafish (87%), Xenopus laevis (95%), Chicken (97%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high-energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family and exists as two isoenzymes: sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms—dimers and octamers—in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs shared among some nuclear genes encoding mitochondrial proteins, which may be essential for coordinated activation during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.