Thermo Fisher Scientific Phospho-Chk2 (Thr383) Polyclonal Antibody
Chk2 단백질의 Thr383 인산화 상태를 특이적으로 검출하는 rabbit polyclonal antibody. Western blot, IHC, ICC/IF에 적합. 인간, 마우스, 랫트 반응성. PBS/glycerol buffer, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human CHEK2 (Accession O96017), corresponding to amino acid residues around phosphorylated Thr383. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816187 |
Product Specific Information
This antibody detects endogenous levels of Chk2 only when phosphorylated at Threonine 383.
Target Information
CHEK2 (CHK2) is a serine/threonine kinase involved in the DNA damage checkpoint pathway. Mutations in CHK2 are associated with various cancers. CHK2 is activated by DNA damage and phosphorylates several modulators of cell cycle control, including tumor suppressor proteins. It can also phosphorylate BRCA1, aiding in survival after DNA damage. CHK2 is considered a tumor suppressor, and mutations in the CHK2 gene are linked to Li-Fraumeni syndrome, sarcomas, breast cancer, and brain tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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