Thermo Fisher Scientific Phospho-EGFR (Thr693) Polyclonal Antibody
EGFR의 Thr693 인산화 부위를 특이적으로 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 액상 형태로 -20°C 보관. 암 관련 연구 및 EGFR 신호전달 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human EGFR (Accession P00533), corresponding to amino acid residues around phosphorylated Thr693 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816190 |
Product Specific Information
This antibody detects endogenous levels of EGFR only when phosphorylated at Thr693, which site has been historically referenced as Thr669.
Target Information
EGFR (epidermal growth factor receptor) is a receptor tyrosine kinase that mediates signaling in response to various growth factors. Overexpression of EGFR has been linked to numerous cancers and serves as a target for both biological and small-molecule therapeutics.
The EGFR gene is located on chromosome 7 in humans and belongs to the HER/ERbB family, which includes ERbB2, ERbB3, and ERbB4.
EGFR is a transmembrane receptor; ligand binding (e.g., EGF, TGF-α) induces dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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