Thermo Fisher Scientific SATB1 Polyclonal Antibody
SATB1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, ICC/IF, Flow Cytometry에 적합. 인간 및 마우스 시료 반응성, 고순도 액상 형태, 4°C 또는 -20°C 보관. 연구용으로만 사용.
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Thermo Fisher Scientific SATB1 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within N-terminal human SATB1 (aa 1–763) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS (pH 7.4) with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2913219 |
Product Specific Information
- Positive Control: Mouse thymus tissue lysate, SH-SY-5Y, Jurkat
- Subcellular Location: Nucleus
Target Information
SATB1 (DNA-binding protein SATB1) is a crucial silencing factor involved in X inactivation mediated by Xist RNA during embryogenesis and lymphoma development. It binds to AT-rich DNA sequences and nuclear matrix-associated regions, recognizing the sugar-phosphate backbone of double-stranded DNA.
SATB1 acts as a transcriptional repressor regulating nuclear and viral gene expression in a phosphorylation- and acetylation-dependent manner. It plays a key role in chromatin-loop remodeling and T-cell maturation (CD8SP).
SATB1-binding sites are preferentially targeted by HIV-1 integration machinery, and HIV-1 Tat may counteract SATB1-mediated repression of IL1 and IL2RA in T-cells by binding to the same domain as HDAC1.
Usage Note
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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