Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), PE, eBioscience
인간 CD127(IL-7Rα)을 인식하는 eBioRDR5 단클론 항체로, PE 형광 표지되어 있습니다. Flow cytometry에 최적화되어 있으며, IL-7 결합에 의해 특이적으로 차단됩니다. T 세포 및 조절 T 세포 연구에 적합합니다.
- 카탈로그번호
- 12-1278-4x (2개 옵션)
- 판매단위
- pk
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Thermo Fisher Scientific CD127 Monoclonal Antibody (eBioRDR5), PE, eBioscience
Applications and Tested Dilutions
- Immunohistochemistry (Frozen): Not specified
- Flow Cytometry: 5 µL (0.06 µg)/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBioRDR5 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10853334 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 561, 647, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV series (496–805)
- Brilliant Violet series (421–786)
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue, Red, UV, Violet, Yellow series
- PE-Cyanine5, 5.5, 7
- PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright series (436–780)
- Custom conjugation available
Product Specific Information
The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha).
CD127 forms a complex with CD132 (common gamma chain) to create the IL-7 receptor (IL-7R). It is a type I glycoprotein (75–80 kDa) expressed on immature B cells, thymocytes, and most mature T cells, with transient down-regulation upon activation.
IL-7 binding triggers signal transduction via tyrosine kinase pathways (Jak/STAT), essential for lymphocyte development and T-cell homeostasis. CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (Tregs), and low CD127 expression enriches for Foxp3+ suppressive Tregs.
Binding of eBioRDR5 to PBMCs is blocked by recombinant human IL-7.
This antibody is validated for flow cytometry, pre-titrated for use at 5 µL (0.06 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).
Excitation: 488–561 nm; Emission: 578 nm; Compatible lasers: Blue, Green, Yellow-Green.
Post-manufacturing filtration: 0.2 µm.
Target Information
CD127 (IL-7Rα) is a glycoprotein regulating lymphopoiesis. The IL-7 receptor is an α/γ heterodimer, with the γ-chain shared with IL-2R.
CD127 signaling promotes proliferation of B cell precursors, thymocytes, and T cells.
Dysfunction of CD127 is associated with severe combined immunodeficiency (SCID) phenotypes.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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