Thermo Fisher Scientific NCF4 Monoclonal Antibody (OTI6H9), TrueMAB
NCF4 단백질을 인식하는 단클론 항체로, Human, Mouse, Rat에 반응합니다. Western blot에 사용 가능하며, HEK293T 세포에서 발현된 full-length human recombinant protein을 면역원으로 사용했습니다. 동결건조 형태로 제공되며, -20°C에서 보관합니다.
- 카탈로그번호
- CF809224
- 판매단위
- pk
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Applications
Tested Application
- Western Blot (WB): 1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6H9 |
| Immunogen | Full length human recombinant protein of human NCF4 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The NCF4 protein is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase complex, crucial for host defense mechanisms.
It is predominantly expressed in myeloid lineage cells and forms complexes with NCF2/p67-phox and NCF1/p47-phox, interacting with RAC1 to activate flavocytochrome b upon stimulation.
The PX domain binds phospholipid products of PI(3) kinase, implicating NCF4 in PI(3) kinase-mediated signaling. Phosphorylation of NCF4 negatively regulates enzyme activity.
Alternative splicing results in distinct isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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