Thermo Fisher Scientific Myeloperoxidase/MPO Recombinant Mouse Monoclonal Antibody (rMPO, 6904)
Recombinant mouse monoclonal antibody against human Myeloperoxidase (MPO). Validated for WB and IHC (Paraffin). Produced in HEK293 cells, purified via Protein A/G, supplied as liquid at 200 µg/mL. Suitable for research on MPO-related diseases.
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- 4353-MSM4-P1
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rMPO, 6904 |
| Immunogen | Recombinant fragment (around aa150–250) of human MPO protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For immunohistochemistry (PFA fixed):
- Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation and is a major component of neutrophil azurophilic granules. It is produced as a single-chain precursor that is cleaved into light and heavy chains. The mature MPO forms a tetramer (2 light + 2 heavy chains). MPO generates hypohalous acids crucial for neutrophil microbicidal activity.
Diseases associated with MPO include Myeloperoxidase Deficiency and Familial Alzheimer’s Disease type 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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