Thermo Fisher Scientific BAF53A Recombinant Rabbit Monoclonal Antibody (5H3L6)
BAF53A 단백질을 인식하는 재조합 토끼 단클론 항체로, Western blot 및 면역세포염색에 적합. 높은 특이성과 재현성을 제공하며, 로트 간 일관성이 우수함. 인간, 생쥐, 랫드에 반응하며 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunocytochemistry (ICC/IF) | 2 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 5H3L6 |
| Immunogen | Peptide corresponding to human BAF53A (aa124–142) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2665307 |
Product Specific Information
This antibody is predicted to react with Monkey, Pig, and Rat.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Enhanced specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
BAF53A is a member of the actin-related protein (ARP) family, sharing significant sequence identity with conventional actins. Both actins and ARPs possess an ATP-binding cleft as a common structural feature.
ARPs participate in cellular processes such as vesicular transport, spindle orientation, nuclear migration, and chromatin remodeling. BAF53A encodes a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex, functionally related to the SWI/SNF complex in yeast and Drosophila. This complex facilitates transcriptional activation by counteracting chromatin-mediated repression.
Together with beta-actin, BAF53A is required for maximal ATPase activity of BRG1 and for chromatin/matrix association of the BAF complex. Three transcript variants encoding two protein isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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