Thermo Fisher Scientific POLA1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human POLA1. Recombinant protein control fragment (Product #RP-92178). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:POLA1,uniProtId:P09884-1,ncbiNodeId:9606,antigenRange:263-381,antigenLength:1462,antigenImageFileName:PA5-51784_POLA1_P09884-1_Rabbit.svg,antigenImageFileNamePDP:PA5-51784_POLA1_P09884-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2645742

Product Specific Information

Immunogen sequence: VDLEPMAAKA WDKESEPAEE VKQEADSGKG TVSYLGSFLP DVSCWDIDQE GDSSFSVQEV QVDSSHLPLV KGADEEQVFH FYWLDAYEDQ YNQPGVVFLF GKVWIESAET HVSCCVMVK

Highest antigen sequence identity to the following orthologs: Mouse - 75%, Rat - 76%.

Target Information

DNA replication, recombination and repair, all of which are necessary for genomic stability, require the presence of exonucleases. In DNA replication, these enzymes are involved in the processing of Okazaki fragments, whereas in DNA repair they function to excise damaged DNA fragments and correct recombinational mismatches. These exonucleases include the family of DNA polymerases. DNA pol alpha, beta, delta and epsilon are involved in DNA replication and repair. DNA pol delta and DNA pol epsilon are multisubunit enzymes, with DNA pol delta consisting of two subunits: p125, which interacts with the sliding DNA clamp protein PCNA; and p50. The nuclear-encoded DNA pol gamma is the only DNA polymerase required for the replication of the mitochondrial DNA. DNA pol zeta is ubiquitously expressed in various tissues and mediates the cellular mechanism of damage-induced mutagenesis. DNA pol theta is a DNA polymerase-helicase that binds ATP and is involved in the repair of interstrand crosslinks.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.