Thermo Fisher Scientific H3K27me1 Polyclonal Antibody
H3K27me1, Me2, Me3 변형을 인식하는 Rabbit Polyclonal 항체로 Western blot, IP, Dot blot에 사용 가능. Human, Mouse, Rat, C. elegans 반응성. 액상 형태로 -20°C 보관, 동결/해동 반복 피해야 함. 크로마틴 연구 및 후성유전학 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunoprecipitation (IP) | 1:1,000 |
| Dot Blot (DB) | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans, Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone H3 K27 methyl Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide surrounding the K27 me3 site of human Histone H3. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.83 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody recognizes the Me1, Me2, and Me3 forms of Lys27 in human Histone H3. BLAST analysis indicates cross-reactivity with Human, Mouse, Rat, and C. elegans based on 100% sequence homology. Cross-reactivity with Histone H3 [methyl Lys27] from other sources has not been determined.
Target Information
The nucleosome consists of 146 bp of DNA wrapped around an octamer of histone proteins (H2A, H2B, H3, and H4). Histone proteins are covalently modified at specific residues, mainly in the N-terminal tail, through processes such as acetylation, methylation, SUMOylation, ubiquitinylation, arginine methylation, citrullination, serine/threonine phosphorylation, and proline isomerization.
Lysine residues can be mono-, di-, or tri-methylated, and arginine residues can be mono- or dimethylated (symmetrically or asymmetrically). These modifications are temporally and cell-specifically regulated, influencing gene activation or repression.
- H3K4Me3 is associated with transcriptional activation at promoter regions.
- H3K4Me1 is found at enhancer sequences.
- H3K9Me1, H3K27Me1, and H4K20Me1 are linked to gene activation.
- H3K9Me2/Me3, H3K27Me2/Me3, and H4K20Me2/Me3 are linked to gene repression.
Monomethylation of H4K20 by SET8 is essential for genome replication and stability. Loss of this mark leads to DNA breaks and p53 activation, preventing abnormal mitosis. In mammalian stem cells, Xist expression inhibits H4K20me1 formation, demonstrating a link between chromatin regulation and stem cell differentiation.
This antibody is suitable for research in Chromatin Biology, Epigenetics, Chromatin Modifiers, Histones, Modified Histones, and Phospho-specific studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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