Thermo Fisher Scientific NEFL Polyclonal Antibody
Rabbit polyclonal antibody targeting human NEFL protein for WB and IHC applications. High specificity recombinant immunogen-based product. Supplied as a liquid, unconjugated form with 0.1 mg/mL concentration. Ideal for neuronal structure and neurofilam...
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Thermo Fisher Scientific NEFL Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000–1:2,500 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human NEFL. Recombinant protein control fragment (Product #RP-104309). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789671 |
Product Specific Information
Immunogen sequence:
SSFSYEPYYS TSYKRRYVET PRVHISSVRS GYSTARSAYS SYSAPVSSSL SVRRSYSSSS GSLMPSLENL DLSQVAAISN DLKSIRTQEK AQLQDLNDRF
Target Information
Neurofilaments are intermediate filament proteins found specifically in neurons, composed mainly of three major proteins: NF-L, NF-M, and NF-H. NF-L is the light or low molecular weight microfilament subunit (~70 kDa). NF-H is the heavy or high molecular weight microfilament subunit (180–220 kDa). These proteins are crucial for maintaining neuronal caliber and are expressed in a tissue-specific and developmentally regulated manner.
Additional Information
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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