Thermo Fisher Scientific ATG4B Monoclonal Antibody (6C3C5)
ATG4B 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot 및 Flow cytometry에 사용 가능. 인간 시료 반응성 확인됨. Protein G 정제 및 PBS buffer에 0.05% sodium azide 포함. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Flow Cytometry (Flow)
- Tested Dilution: 1:200–1:400
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 6C3C5 |
| Immunogen | Purified recombinant fragment of human ATG4B (aa: 1–221) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long-term storage, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898365 |
Product Specific Information
This antibody has been tested in indirect ELISA.
Target Information
Cysteine protease ATG4B is an enzyme encoded by the ATG4B gene. Autophagy is a critical cellular homeostatic process controlling turnover of damaged organelles and proteins.
ATG4B acts as a deubiquitin-like protease with dual roles in the autophagy core machinery.
It cleaves the C-terminal amino acid of ATG8 family proteins (MAP1LC3, GABARAPL1, GABARAPL2, GABARAP) to expose a C-terminal glycine, which is essential for ATG8 conjugation to phosphatidylethanolamine (PE) and membrane insertion.
ATG4B is mainly expressed in skeletal muscle, brain, heart, liver, and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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