Thermo Fisher Scientific PGP9.5 Monoclonal Antibody (B1-5-6)
PGP9.5 단백질을 인식하는 Mouse IgG2a 단클론 항체로, 신경세포 특이적 표지에 활용 가능. WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합. 고순도 Affinity Chromatography 정제, 안정적 액상 형태로 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | B1-5-6 |
| Immunogen | Synthetic peptide within Human PGP9.5 aa 174–223 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 0.2% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848821 |
Product Specific Information
Positive Control: A172, SHG-44, A549, SH-SY5Y, rat cerebellum tissue, human kidney tissue, human pancreas tissue, mouse brain tissue.
Target Information
PGP9.5 (Protein gene product 9.5, UCH-L1, PARK5) is a neuron-specific protein distinct from neuron-specific enolase. It has a molecular weight of 27 kDa and belongs to the ubiquitin carboxyl-terminal hydrolase family (peptidase family C12). PGP9.5 exhibits both hydrolase and ligase activities, generating ubiquitin monomers from small C-terminal adducts. It is found in neurons and nerve fibers of the central and peripheral nervous system, neuroendocrine cells, renal tubules, spermatogonia, Leydig cells, and ovarian tissues. Overexpression is associated with non-small cell lung cancer, while decreased expression correlates with Huntington and Alzheimer diseases. PGP9.5 serves as a neuronal marker and is valuable in neurodegenerative disorder research, including Parkinson disease studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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