Thermo Fisher Scientific IL-32 Polyclonal Antibody
Human IL-32 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western Blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 고순도 항원 친화 크로마토그래피로 정제. 냉장 보관 시 안정적이며 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5–4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–10 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A 15 amino acid peptide from near the carboxy terminus of human IL-32 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11157875 |
Product Specific Information
A suggested positive control is human spleen tissue lysate.
PA5-20221 can be used with blocking peptide PEP-0334.
Target Information
Interleukin-32 (IL-32) was initially identified as a transcript (NK4) selectively expressed in lymphocytes and NK cells, with expression increased following activation by IL-2. Later, it was re-isolated from an IL-18-treated lung carcinoma cell line and renamed IL-32.
IL-32 is unique in that it does not share sequence homology with known cytokine families and is highly expressed in immune tissues, existing in at least four spliced isoforms.
Treatment of human monocytic and mouse macrophage cells with IL-32 induces several proinflammatory cytokines such as TNF-α, IL-8, and MIP-2. It is also induced in human peripheral lymphocytes after mitogen stimulation and in epithelial cells by IFN-γ, suggesting a role in autoimmune and inflammatory diseases such as rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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