Thermo Fisher Scientific Fentanyl Polyclonal Antibody

Applications

Tested Dilution

Publications

ELISA (ELISA)

10 µg/mL

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Sheep / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Fentanyl-BSA

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

13.26 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1090127

Product Specific Information

Working Concentration: 1/500. Sensitivity: 10 ng/mL Fentanyl produces 84% inhibition in a competitive ELISA employing Fentanyl polyclonal antibody.

Target Information

Fentanyl is a synthetic, lipophilic phenylpiperidine opioid agonist with analgesic and anesthetic properties. Fentanyl selectively binds to the mu-receptor in the central nervous system (CNS) thereby mimicking the effects of endogenous opiates. Stimulation of the mu-subtype opioid receptor stimulates the exchange of GTP for GDP on the G-protein complex and subsequently inhibits adenylate cyclase. This results in a decrease in intracellular cAMP and leads to a reduction in the release of neurotransmitters such as substance P, GABA, dopamine, acetylcholine and noradrenaline. The analgesic effect of fentanyl is likely due to its metabolite morphine, which induces opening of G-protein-coupled inwardly rectifying potassium (GIRK) channels and blocks the opening of N-type voltage-gated calcium channels, thereby resulting in hyperpolarization and reduced neuronal excitability. [National Center for Biotechnology Information. PubChem Compound Database; CID=3345].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.