Thermo Fisher Scientific HIF1 alpha Polyclonal Antibody
HIF1 alpha 단백질을 인식하는 토끼 폴리클로날 항체로, Western blot과 IHC에 사용 가능. 인간 시료에 반응하며, 저산소 반응 관련 연구에 적합. 동결건조 형태로 제공되며, 4°C 또는 -20°C에서 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | – |
| Immunohistochemistry (IHC) | 10–50 µg/mL | – |
| Miscellaneous PubMed (Misc) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the internal region of human HIF1 alpha (HIF1A, MOP1, Hypoxia-inducible factor 1 alpha) conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
- The antigen shares 94% identity with rat and mouse sequences.
- Specificity: HIF1A.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer consisting of alpha and beta subunits, both belonging to the basic-helix-loop-helix Per-aryl hydrocarbon receptor nuclear translocator-Sim (PAS) family. It regulates the adaptive response to hypoxia by activating transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, VEGF, and others involved in oxygen delivery and metabolic adaptation.
HIF1-alpha also regulates hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. Under hypoxia, it interacts with p53 and undergoes MDM2-mediated ubiquitination and degradation. It also induces NOX4 to maintain ROS levels and support hypoxia-induced proliferation.
In humans, HIF1A is located on chromosome 14q. The C-terminal of HIF1A binds to p300, forming p300/CBP-HIF complexes that induce hypoxia-responsive genes, including VEGF.
Hypoxia plays a critical role in diseases such as myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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