Thermo Fisher Scientific His tag Polyclonal Antibody
His tag 단백질 검출용 토끼 다클론 항체로 Western blot 및 IHC에 적합. 합성 hexa-His 항원을 사용하여 제작됨. 비결합형, 동결건조 형태로 제공되며 장기 보관 시 -20°C 권장. 재조합 단백질의 정제 및 검출 연구에 활용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous (PubMed) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic hexa His (HHHHHH) conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 150 µL of sterile water.
- Centrifuge to remove any insoluble material.
- Specificity: His tag (HHHHHH).
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
This tag is commonly expressed at the N- or C-terminal regions of recombinant proteins to enable purification by immobilized metal affinity chromatography (IMAC).
Epitope tagging allows detection of recombinant proteins for which no specific antibody exists.
His-tagged proteins are purified using metal chelate chromatography due to the histidine cluster’s metal-binding properties.
The affinity between the His-tag motif and Ni²⁺ is strong and selective, allowing purification to homogeneity on Ni²⁺-NTA adsorbents.
His tag-specific antibodies are used for detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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