Thermo Fisher Scientific His tag Polyclonal Antibody
His tag(HHHHHH)에 특이적인 sheep polyclonal antibody로, WB와 IHC에 사용 가능. 재조합 단백질의 검출 및 정제에 적합하며, 동결건조 형태로 제공. 4°C 또는 -20°C에서 보관하며, 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:300–1:2,000 | - |
| Immunohistochemistry (IHC) | 1:300–1:2,000 | - |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic hexa His (HHHHHH) conjugated to blue carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Reconstitute in 150 µL of sterile water. Centrifuge to remove any insoluble material.
Specificity: His tag (HHHHHH).
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH).
It is commonly expressed at the N- or C-terminus of recombinant proteins to facilitate purification via immobilized metal affinity chromatography (IMAC).
His tag-specific antibodies are used for detection or co-immunoprecipitation of tagged proteins.
Histidine residues bind selectively to Ni²⁺, enabling efficient purification using Ni²⁺-NTA adsorbents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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