Thermo Fisher Scientific NEFM Monoclonal Antibody (RMO 14.9)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

-

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50 - 1:100

-

ELISA (ELISA)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 2 publications 2 publications

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rabbit, Rat

Published species

Human, Mouse, Pig, Rat, Rodent, Sheep

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

RMO 14.9

Immunogen

Rat NF-M.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533154

Target Information

Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs, in most vertebrates, are composed of three different polypeptide chains with different molecular weights - neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments are critical for size and cytoskeletal architecture of axons, and are mediated, in part, by the highly phosphorylated tail domain of this protein. NF-M phosphorylation and O-GlcNAcylation are regulated reciprocally and affect its translocation and filament formation and function. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.