Thermo Fisher Scientific NEFM Monoclonal Antibody (RMO 14.9)
신경세포 특이적 중간 필라멘트 단백질 NF-M을 인식하는 마우스 단클론 항체. Western blot, IHC, ICC 등 다양한 응용에 사용 가능. 여러 종에 반응하며 Protein A로 정제된 액상 형태. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 | - |
| ELISA | Assay-dependent | - |
| Miscellaneous PubMed (Misc) | - | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chicken, Human, Mouse, Rabbit, Rat |
| Published Species | Human, Mouse, Pig, Rat, Rodent, Sheep |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | RMO 14.9 |
| Immunogen | Rat NF-M |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2533154 |
Target Information
Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, particularly in large axons. NFs in vertebrates consist of three polypeptide chains: neurofilament medium protein (NF-M), high (NF-H), and light protein (NF-L). These share a coiled-coil core domain but differ in terminal sequences, especially in the C-termini, which form flexible extensions linking NFs to each other and other cytoplasmic elements.
NF-M tail-mediated interactions are critical for axonal size and cytoskeletal architecture, influenced by phosphorylation and O-GlcNAcylation. Antibodies against NF subunits serve as useful neuronal markers due to their abundance, neuron-specific expression, and biochemical stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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