Thermo Fisher Scientific MAPK7 Monoclonal Antibody (OTI1G1), TrueMAB
MAPK7(ERK5) 단백질을 표적하는 Mouse Monoclonal Antibody로, WB, ICC/IF, Flow Cytometry에 사용 가능. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공되며, 재용해 후 1 mg/mL 농도로 사용. 세포 신호전달 및 발달 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1G1 |
| Immunogen | Full length human recombinant protein of human MAPK7 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAPK7 (ERK5) is a serine/threonine kinase belonging to the MAP kinase family, involved in cellular processes such as proliferation, differentiation, transcription regulation, and development.
Activated by MAP2K5/MEK5, MAPK7 translocates to the nucleus upon extracellular stimulation to regulate gene expression by phosphorylating transcription factors.
Gene deletion studies in mice reveal its essential role in vascular and cardiac development. ERK5 is highly expressed in heart, placenta, lung, kidney, and skeletal muscle, but not detectable in liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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