Invitrogen™

Subcloning Efficiency™ DH5α Competent Cells

Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1자세히 알아보기

Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA. Subcloning Efficiency DH5α Competent Cells are an economical solution for routine subcloning procedures or any general application where DNA for transformation is not limiting.

Features of Subcloning Efficiency DH5α Competent Cells
• Designed for general, low throughput, everyday use
• Standard laboratory strain useful for general cloning applications

Ideal for routine subcloning procedures
Subcloning Efficiency DH5α Competent Cells are recommended for routine subcloning of genes into plasmid vectors. 500 μL of chemically competent cells are pre-aliquoted in tubes for ten reactions using 50 μL. These cells yield >1 x 106 transformants/μg control DNA per 50 μL reaction. Subcloning Efficiency DH5α Competent Cells are not suitable for the generation of cDNA libraries.

Flexible cloning capabilities
Benefits of Subcloning Efficiency DH5α Competent Cells include:
• lac_ZΔM15 for blue/white color screening of colonies on plates containing X-gal or Bluo-gal
• _rec_A1 that ensures increased insert stability and prevents unwanted recombination
• _end_A1 that improves the yield and quality of plasmid DNA prepared from minipreps
• Supports replication of _M13mp vectors but not plaque formation

Genotype
F- Φ80_lac_ZΔM15 Δ(_lac_ZYA-_arg_F) U169 _rec_A1 _end_A1 _hsd_R17(rk-, mk+) _pho_A _sup_E44 _thi_-1 _gyr_A96 _rel_A1 λ-

Genetic marker descriptions

Find the strain and format that you need
We offer other DH strains in chemically competent and electrocompetent cell formats to meet your specific needs.
Strains are available for challenging cloning applications such as library preparation or cloning unstable DNA.
SOC medium for E. coli recovery after transformation is available, as well as other ready-to-use bacterial growth formats.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝No

형질전환 효율 수준Subcloning Efficiency (106 to 107 cfu/μg)

항생제 내성 박테리아No

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

형식Tube

제품라인Subcloning Efficiency™

수량4 x 500 μL

Unit SizeEach