Invitrogen™

Library Efficiency™ DH5α Competent Cells

Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning자세히 알아보기

Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are able to achieve transformation efficiencies of >1 x 108 cfu/μg control DNA per 100 μL reaction.

Library Efficiency DH5α Competent Cells are recommended for routine cloning of genes into large plasmids and are suitable for difficult clone constructions, such as blunt-end ligations or cloning using limited amount of DNA.

The strain has the _lac_ZΔM15 genotype, allowing blue-white screening on plates containing either X-Gal or Bluo-Gal. It also carries the _rec_A1 mutation, which helps reduce the rate of recombination while propagating plasmid DNA, and the _end_A1 mutation that makes DH5α cells an excellent choice for amplifying plasmid DNA for subsequent extraction and purification. DH5α cells are capable of being transformed efficiently with large plasmids and can serve as a host for the M13mp cloning vectors if a lawn of DH5αF′IQ, for example, is provided to allow plaque formation.

Library Efficiency DH5α Competent Cells offer:
• Transformation efficiencies of >1 x 108 cfu/μg
• _lac_ZΔM15 marker that provides α-complementation of the β-galactosidase gene allowing blue/white screening on agar plates containing X-Gal or Bluo-Gal
• Plasmid DNA maintained with minimum recombination events due to _rec_A1 mutation
• _end_A1 mutation that enables increased plasmid yield and quantity
• _hsd_R17 genotype that allows efficient transformation of cloned unmethylated DNA from PCR

Genotype
F- Φ80_lac_ZΔM15 Δ(_lac_ZYA-_arg_F) U169 _rec_A1 _end_A1 _hsd_R17 (rk-, mk+) _pho_A _sup_E44 λ-_thi_-1 _gyr_A96 _rel_A1

Find the strain and format that fits your needs
DH5α strain and its derivatives are available with a variety of transformation efficiencies and in both electrocompetent and chemically competent formats.
MAX Efficiency DH5αF´IQ Competent Cells are available in chemically competent format.
DH5α-T1R competent cells are available in MultiShot format for high throughput applications.
Explore bacterial growth media formats.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝No

형질전환 효율 수준Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)

항생제 내성 박테리아No

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

형식Tube

제품라인Library Efficiency™

수량5 x 200 μL

Unit SizeEach