Invitrogen™

MAX Efficiency™ DH10B Competent Cells

MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain자세히 알아보기

MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency, making them ideal for generating plasmid libraries, an ability to take up and maintain large plasmids (seen with plasmids of 150 kb in size), and a lack of methylation-dependent restriction systems.

Benefits of MAX Efficiency DH10B Competent Cells
• Ability to maintain large DNA plasmids
• Resistant to the effects of ligase and ligase buffer and tolerant of small amounts of undiluted ligation reactions
• Suitable for the construction of cDNA or genomic DNA libraries using plasmid-derived vectors

Universal strain for everyday applications
The presence of the mcrA genotypic marker and the deletion of mcrBC and mrr makes this strain suitable for cloning DNA that contains methylcytosine and methyladenine. Therefore, genomic DNA, both prokaryotic and eukaryotic, can be cloned efficiently in DH10B.

Versatile DH10B genotype features
• lac_Z∆M15 for blue/white screening of recombinant clones
• Elimination of _mcr_A, _mcr_BC, _mrr, and _hsd_RMS restriction systems to allow construction of more representative genomic libraries
• endA1 mutation to increase plasmid yield and quantity

Genotype
F–mcr_A Δ(_mrr_-_hsd_RMS-_mcr_BC) φ80_lac_ZΔM15 Δ_lac_X74 _rec_A1 _end_A1 _ara_D139 Δ(_ara-leu)7697 _gal_U _gal_K λ–_rps_L(StrR) _nup_G

Genetic marker descriptions

Find the strain and format that fits your need
DH10B Cells are available in both electrocompetent and chemically competent formats.
MegaX DH10B T1R Electrocomp Cells have the highest transformation efficiency (>3 x 1010 cfu/μg plasmid DNA).
Multishot formats are available for high throughput applications.

사양

제품 유형Chemically Competent Cells

에프에피솜 포함No

플라스미드 품질 개선Yes (endA1)

메틸화 DNA 클로닝Yes (mcrA)

형질전환 효율 수준High Efficiency (>1 x 109 cfu/μg)

항생제 내성 박테리아Yes (Streptomycin)

불안정 DNA 클로닝Not suitable for cloning unstable DNA

블루/화이트 스크리닝Yes (lacZΔM15)

고처리량 호환성Low

비메틸화 DNA 준비No

재조합 감소Yes (recA1)

배송 조건Dry Ice

T1 Phage - 저항성(tonA)No

종E. coli (K12)

형식Tube

제품라인MAX Efficiency™

수량5 x 200 μL

Unit SizeEach