Thermo Fisher Scientific NEFL Monoclonal Antibody (2F11)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

-

Immunohistochemistry (IHC)

-

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:200

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:25-1:200

-

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Avian, Rabbit, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2F11

Immunogen

Human neurofilament preparation if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:NEFL,uniProtId:P07196-1,ncbiNodeId:9606,antigenRange:1-543,antigenLength:543,antigenImageFileName:MA1-06803_NEFL_P07196-1_House_mouse.svg,antigenImageFileNamePDP:MA1-06803_NEFL_P07196-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_560286

Product Specific Information

MA1-06803 detects neurofilament 70 kDa in human samples.

MA1-06803 has sucessfully been used in Western blotting, and immunohistochemistry procedures. By Western blot it detects a 70 kDa band representing neurofilament ~70 kDa.

The MA1-06803 immunogen is a human neurofilament preparation.

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.